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Contents

Overview

RNA sample loading buffer is formulated for use in RNA sample preparation for denaturing formaldehydeagarose gel electrophoresis. The solution contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required.

Materials


Note: This solution contains ethidium bromide that can interfere with transfer of RNA to nylon or nitrocellulose membranes.

Procedure

Add loading buffer to sample in ratio of 1:2 to 1:5. Just before loading, heat to 65 °C for 10 minutes, then chill on ice.

References

Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory (1989), p. 7.43-7.45, 7.51

Contact

Product No. R 4268


or instead, discuss this protocol.