NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. On September 1st 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.




  1. After electrophoresis, remove the gel from the gel box and plates using a gel separator
  2. Place it in a clean plastic container and cover with ultrapure water
  3. Microwave the gel on high for 1 minute until the solution almost boils (do not overheat)
  4. Shake the gel on a shaker for 2 minute and discard the water
  5. Repeat steps 3 and 4 two more times
  6. After the last wash, add 30 ml of SimplyBlue™ Coomassie protein stain and microwave on high for 45 seconds to 1 minute until the solution almost boils
  7. Shake the gel on a shaker for 10 minutes
  8. Wash the gel in 100 ml of ultrapure water for 10 minutes on a shaker
  9. Add 20 ml of 20% NaCl for at least 5 minutes
  10. Examine gel on a white paper towel for blue-stained protein bands
  11. Discard the gel in the Biohazard waste bin



discuss this protocol.