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Contents

Materials

Procedure

  1. After electrophoresis, remove the gel from the gel box and plates using a gel separator
  2. Place it in a clean plastic container and cover with ultrapure water
  3. Microwave the gel on high for 1 minute until the solution almost boils (do not overheat)
  4. Shake the gel on a shaker for 2 minute and discard the water
  5. Repeat steps 3 and 4 two more times
  6. After the last wash, add 30 ml of SimplyBlue™ Coomassie protein stain and microwave on high for 45 seconds to 1 minute until the solution almost boils
  7. Shake the gel on a shaker for 10 minutes
  8. Wash the gel in 100 ml of ultrapure water for 10 minutes on a shaker
  9. Add 20 ml of 20% NaCl for at least 5 minutes
  10. Examine gel on a white paper towel for blue-stained protein bands
  11. Discard the gel in the Biohazard waste bin

References

Contact


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