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Contents

MDCK cell culture

JCC 2/25/11

From James Nelson’s lab, cells from Lance Kam's lab

Materials

DMEM prep

  1. Add 5.0 g DMEM to 475 ml MilliQ water.
  2. Stir to dissolve
  3. Add 0.5 g of sodium bicarbonate.
  4. pH of media should be 7.0 - use concentrated HCl to adjust
  5. Bring up to final volume of 500 ml.
  6. Filter sterilize with a 0.22 μm filter.

Working media

500 ml DMEM (serum-free) 50 ml FBS 5 ml Pen/Strep